Detergent screening of the human voltage-gated proton channel using fluorescence-detection size-exclusion chromatography
نویسندگان
چکیده
منابع مشابه
The gating mechanism of human voltage - gated proton channel Hv 1 studied by fluorescence technology
[2]Research Progress(10.5 points) In this project, we investigated the role of Nand C-terminal domain of human voltage-gated proton channel Hv1 in the gating mechanism using fluorescence technology. The plan in details is as follows: (1) To determine the interaction between Nand C-terminal domains, the N-terminal domain was cloned, expressed and purified. (2) The interaction between Nand C-term...
متن کاملFluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins.
Formation of well-ordered crystals of membrane proteins is a bottleneck for structure determination by X-ray crystallography. Nevertheless, one can increase the probability of successful crystallization by precrystallization screening, a process by which one analyzes the monodispersity and stability of the protein-detergent complex. Traditionally, this has required microgram to milligram quanti...
متن کاملA fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening.
Optimization of membrane protein stability under different solution conditions is essential for obtaining crystals that diffract to high resolution. Traditional methods that evaluate protein stability require large amounts of material and are, therefore, ill suited for medium- to high-throughput screening of membrane proteins. Here we present a rapid and efficient fluorescence-detection size-ex...
متن کاملVoltage-gated proton channel in a dinoflagellate.
Fogel and Hastings first hypothesized the existence of voltage-gated proton channels in 1972 in bioluminescent dinoflagellates, where they were thought to trigger the flash by activating luciferase. Proton channel genes were subsequently identified in human, mouse, and Ciona intestinalis, but their existence in dinoflagellates remained unconfirmed. We identified a candidate proton channel gene ...
متن کاملOrganic matter from biofilter nitrification by high performance size exclusion chromatography and fluorescence excitation-emission matrix
A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2014
ISSN: 0961-8368
DOI: 10.1002/pro.2492